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doi: 10.1038/icb.2017.56 113 Nyul-TothAKozmaMNagyosziPNagyKFazakasCHaskoJet al

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After washing the slices with PBS three times, we incubated them with Alexa Fluor 647 goat anti-rabbit secondary antibody (A-21245, Thermo Fisher Scientific, Waltham, MA, USA), diluted 1:500 in the blocking solution, for 2 hours at room temperature