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Once samples are approved we begin the manufacturing process

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The program of qPCR reaction included 95 C for 10 min, 40 cycles of 95 C for 5 s and 60 C for 15 s

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C408A substitution abolishes the activity of the PDO domain We previously reported that the C408S substitution negatively impacts the thiosulfate production of CstB and that no sulfite can be detected even when the PDO domain remains intact 19

Nguyen HM, Ngoc Le TT, Nguyen AT, Thien Le HN, Pham TT