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The temperature of column oven was set at 40 C, and the injection volume was 1 L

Bacterial contamination, even at low levels undetectable by visual inspection alone, introduces endotoxins that confound experimental results by triggering inflammatory responses independent of the peptide's intended mechanism

These compounds increase SIRT1 expression or activity, collectively improving hepatic lipid metabolism, insulin signaling, oxidative stress, autophagy, and inflammatory responses, highlighting their strong multi-target pharmacological potential

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Savalli et al., 2021