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As maternal deposition constitutes the sole source of minerals available to the developing embryo during incubation, both the quantity and distribution pattern of minerals within the egg are critical determinants of embryonic viability, skeletal development, immune competence, and post-hatch performance

Wang, X

Hum Reprod 1998

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6 Importantly, this method is associated with numerous limitations, including variability between batches, time-consuming requirements, and difficulties in ensuring consistent quality when applied to commercial production settings